Incubator Accessories
Diagnostic Biosystems BCL-2 ONCOPROTEIN Bcl-2 Oncoprotein
This antibody recognizes a protein of 25-26kDa, identified as bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. This antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and small number in which neoplastic cells are bcl-2 negative.
Diagnostic Biosystems UnoVue™ Mouse AP Detection Reagent
UnoVue Mouse AP Detection Reagent is a non-biotin one-step detection reagent suitable for demonstrating antigens in formalin-fixed paraffin-embedded tissues and frozen sections. The UnoVue detection reagents may also be used with blood smears, cytosmears, and cell preparations. UnoVue detection reagents have been developed by directly labeling anti-mouse immunoglobulins with enzymes using a proprietary tandem hyperlabelling technology. This ensures consistent and reproducible immunodetection of mouse antibodies against nuclear, cytoplasmic and membrane antigens in different types of tissues. The single step UnoVue Detection Reagent enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background. UnoVue Mouse AP Detection Reagent is suitable for use with mouse all mouse antibodies, both monoclonal and polyclonal.
Diagnostic Biosystems PURPLE HEMATOXYLIN FOR IHC Purple Hematoxylin for IHC
Hematoxylin is a histological staining reagent suitable for visualization of nuclei in tissue sections. Purple Hematoxylin, is designed for optimal performance when used together with the Mohs HRP-Green Kit. DBS’s Purple Hematoxylin is intended for use in the histologic demonstration of nuclear staining. This staining technique is used to make the critical distinction between a normal nucleus and an abnormal one. With Mohs HRP-Green kit, purple hematoxylin provides better contrast and cellular mophology than tranditional hematoxylin conterstains.
Diagnostic Biosystems EMA Epithelial Membrane Antigen (EMA) E29
This antibody reacts with an antigen of 265‐400 kDa belonging to a heterogeneous group of heavily glycosylated proteins called human milk fat globule proteins. It stains both normal and neoplastic cells. Among normal epithelia, it reacts strongly with mammary epithelium and glandular epithelia but shows a patchy staining with squamous epithelium.\n
Diagnostic Biosystems FITE STAIN Fite's Stain
The Fite’s Stain Kit (For Leprosy and Nocardia) is intended for use in the histological visualization of mycobacterium Lepra bacillus and Nocardia. This kit may be used on formalin-fixed, paraffin-embedded sections.\n
Diagnostic Biosystems IGM-FITC IgM-FITC
This antibody reacts with the Mu chain of human IgM. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may also be used for other applications.
Diagnostic Biosystems PROTEINASE K 25X Proteinase K
Proteinase K is specifically formulated to achieve proteolytic digestion of formalin fixed, paraffin embedded tissue sections prior to performing in situ hybridization procedures.\n\nProteolytic digestion of formalin fixed tissues improves the accessibility of target DNA to biotinylated probes, which results in better in situ staining.
Diagnostic Biosystems AFB AFB
This kit stains acid-fast bacteria and tubercle bacilli of Mycobacterium genus in paraffin tissue sections. Tubercle bacilli and other acid-fast bacteria stain bright red and the background stains light green.
Diagnostic Biosystems Solution Pepsin
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves accessibility of antibodies to tissue antigens.\n\nFixation is one of the most critical aspects of immunostaining. If the antigen is not properly fixed it will be washed out of the specimen. On the other hand, overfixation can cause severe problems such as masking or denaturation of the antigen. Formaldehyde is probably the best all-around fixative, due to its cross-linking characteristics. Length of fixation is very critical to prevent antigen masking.
Diagnostic Biosystems C3C-FITC C3c Complement-FITC
This antibody reacts with the human C3c complement and the C3c part of C3 and C3b. It may cross react with the C3c complement from cat, cow, dog, goat, guinea pig, kangaroo, mink, mouse, rat, sheep, and swine. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may be also used for other applications.
Diagnostic Biosystems ALK PHOS ENHANCER Alkaline Phosphatase Enhancer
This product is used as a signal enhancer in conjunction with alkaline phosphatase based immunostaining systems.\n\nAlkaline Phosphatase, an enzyme derived from bovine intestinal mucosa, is often used as a label for in situ hybridization, immunohistochemistry, Southern and Northern hybridization and DNA sequencing. Detection of this enzyme requires the generation of an insoluble colored reaction end product. Stronger the signal, better the staining. DBS introduces a new stable solution to increase the signal generated by alkaline phosphatase several fold. A single treatment of tissue sections with enhancer before the treatment with substrate/chromogen enables the visualization of difficult to localize antigens. This enhancer works well with Fast Red but can be used with BCIP/NBT and BCIP/INT also. Specimens stained with the above substrates/chromogens can not be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium like C/C Mount (DBS catalog # K002).
Diagnostic Biosystems C3C-FITC C3c Complement-FITC
This antibody reacts with the human C3c complement and the C3c part of C3 and C3b. It may cross react with the C3c complement from cat, cow, dog, goat, guinea pig, kangaroo, mink, mouse, rat, sheep, and swine. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may be also used for other applications.
Diagnostic Biosystems FIBRINOGEN-FITC Fibrinogen-FITC
This antibody reacts with native fibrinogen and with fibrinogen fragments D, E, X, and Y. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may also be used for other applications.